what recession?

People say that Ann Arbor (or UM in particular) is like a giant bubble: whatever is happening outside usually doesn't have too big of an effect on what's inside. We didn't need any financial bailout; the unemployment rate in the Deuce is 7.5%, one of the lowest in Michigan. Professors enjoy job security, because we can't possibly outsource scientific research or our education.

I guess this would be more true if I were still in undergrad.

The lack of money for research is really taking a toll on many of the PIs here; with thesis mentor decision deadlines looming, everyone is scrambling to find a permanent home, but who has the space to take on more students? I've done four rotations, and I enjoyed three of them, wanting to go back and do a thesis, but the labs either have grants pending and won't know until later in the year (when it might be too late to join) or can't afford to take students.

Kinda makes me wonder if this "economic stimulus" is just a myth, since I don't see any of it happening anytime soon.

Grad students are either paid as research assistants or teaching assistants. In MCDB, the teaching requirement is 2 terms, and I think during those two terms, the grad student's stipend doesn't come out of the professor's pocket. So an extra teaching load is another way to join a lab that might not have sufficient funds, but I talked to the MCDB advisor, and she said it was not recommended due to the time spent away from the lab bench, and it'll drag out the time until graduation.

So.... onto rotation #5. There's so much more to learn... just like the first one this past July. The project is definitely interesting: stress hormones and its role in development (how ironic, given my own cortisol levels right now), using Xenopus as a model system.

Never worked with amphibians before... better start reading up on metamorphosis.

Fourth and final

Started my last rotation today.

I was doing pigmentation and adaptation (in particular, starvation) in fruit flies during the previous rotation for January and most of this month, but this new lab is dealing with neuron development. Still in fruit flies, but a whole lot of microscopy and larvae dissecting. Not to mention cell bio... I really need to brush up on cell signaling.

I've had no motivation to do work lately.. maybe it's because it's the middle of "spring break" (applies to undergrads only, unfortunately) or maybe I just want to settle down in a thesis lab and get started. I have a feeling that choosing a thesis lab is gonna be difficult: out of my three past rotations, I'd like to go back to two of them. So right now it's cell fate determination in zebrafish vs. evo-devo in flies. Tenured professor vs. relatively new professor. If this rotation goes well, it'll end up being tenured professor vs. relatively new professor vs. REALLY new professor.

On choosing labs, I'm getting mixed messages. My dad's telling me to go with a tenured professor; someone's who's established in the field. They'll have lots of publications in their name and they'll have an "easier" time getting funds, he tells me. On the flip side, younger PIs have a stronger drive to publish, since they're still establishing themselves in the field and/or are subjected to the tenure review process. Either way though... I know that all the labs I've rotated in so far have good mentors.

In a different note, I didn't realize how many people in grad school are older and more experienced than me; it seems like not too many people came straight from undergrad. Many have had at least one year off from school and have done lab tech work for quite a while. Others are in their upper twenties/lower to mid-30s, are married, or have kids already. It kinda makes me wonder if applying and entering grad school was a hasty decision on my part.

Then again, three years from now I guess it won't make too much of a difference, since everyone's going to have candidacy (hopefully) and working on their projects.

Rotations are exhausting. Must decide by Tax Day...

click here

http://www.nature.com/nature/newspdf/evolutiongems.pdf

'08 in retrospect

I know... I know... it's Christmas Eve and instead of going to another one of those family-friendly holiday parties (read: the one where people my parents' age tell me how much I've grown), I'm sitting here listening to whatever music I have stored on my computer and updating this blog I created. Anyway, the cell bio final (which I was panicking about) turned out to be pretty easy, and I don't have to think about class for another week, so now I intend to forget all about RTKs and G proteins. Oh wait... what about next term's rotations? oh well...

Quite a year in retrospect...originally I thought that graduation would change a lot of things. So many people moving out of AA, getting jobs, off to med school, grad school, or whatever plans they have... clear across the country. I had thought that this past summer would be pretty mellow, since my friends were leaving and grad school, along with a complete shift in priorities, was starting. But in reality, this summer was pretty much one big hurrah interrupted with labwork, locking myself out, and accidentally flooding the zebrafish room. That and drama... not the minor kind that keeps life interesting, but the kind that I haven't seen since middle school. Best song to describe it would prolly be that Maroon 5 song "Tangled."

Grad school in itself is relatively enjoyable; it makes me a little sad that people in PIBS aren't as close knit as the physics grad students I know. The first year physics students have their offices all in this one hallway, and even tho there are assigned desks and rooms, nobody really pays attention, so books, like their owners, are all over the place. It's not just studying either; Michelle says that the boys in the department have already broken a grand total of three ceiling tiles, got reprimanded by both the building manager and older grad students for playing soccer/kickball/football in the hallway, and I guess for just being obnoxious in general. And maybe for also throwing full Nalgene bottles at each other. Gotta love 'em...and their whiteboard of inappropriate quotes.

I think it's nice that even though we're all in grad school and have definitely matured since freshman year of undergrad, we're in no way transcending insane or boring.

Looking forward to next term, since I'm taking development (finally!) and a seminar on teaching in science. Should be fun. Plus my lab rotations will be back in Nat Sci, so maybe a little more time to visit the physics department and promote, er, PREVENT, future ceiling tile destruction. That, and Michelle and I decided that we need to go sledding in the Arb. But since nobody has a sled or toboggan or sorts, we'll use Ben as the human sled. He won't mind.

Hopefully.

obligations...

The postdoc who's training me during rotations was out at a conference the past few days, so I slacked a little in lab. I know that grad students make their own schedule, so they go to lab whenever it's necessary and leave whenever experiments are done. Although a lot of the times I feel like I "need" to be there for the entire 8-hour block, or however long it takes, even if there's nothing to do except sit and wait for something to finish (thank god for the thermocycler).

I'm not entirely used to this med school lab yet... it's been two months! There's two more to go before the end of the rotation, and I'm moving back to Nat Sci in January, but I'm relatively sure now that I want to do a thesis with MCDB...

some natural light would be nice too, I suppose. :-)

Month 1

Cell bio is driving me insane. We're covering stuff on protein transport and we're supposed to read these three papers for discussion, along with answering questions. The problem I'm having is that I understand the gist of the papers, but the questions that we're supposed to answer ask for basically "what were the authors thinking when they did this?" And being papers from Nature, data was usually "not shown," or "in the supplementary material"

I guess another problem is that I write down what I think is the right answer to the question, that nagging "what if this happened" scenario pops up, and then I have to change it.

Or maybe I'm just having an "off" day.

...and I'm gonna go study genetics now.

Week 1

New project: get to be working with embryonic stem cells isolated from mice. There's a paper out there that plated ESCs in media and pretty much "forced" the cells to differentiate into hormone producing cells of the pituitary. So I'll be repeating their experiment; growing cells in a dish, extracting RNA, and doing RT-PCR to find out what kind of markers are expressed in the different stages of development.

Classes just started last week, so the workload hasn't gotten into full swing yet; on the flip side, there's a lot more papers to read. First years take two classes, an ethics seminar, and do lab rotations. Which translates to four papers to read a week, at least.

We'll see how things go once the ethics seminars begin.

Another note.. the new rotation student in my previous lab (zebrafish one) tracked me down in class earlier this week to ask me a question about my lab notebook. He's working on the extension of my rotation project this summer: still trying to map the mi40 gene. But he gets to jump right into the mapping part; I spent my entire summer looking for mi40 hets. Anyways, there's another gene in that lab, mi215, which, when mutated, gives the same phenotype as mi40. The fish don't complement, which means two things are possible:

a. mi40 and mi215 are the same gene
b. they are different genes, but in the same pathway to get a functional eye. So if one of them is faulty, the entire pathway collapses

The new rotation student told me that mapping mi40 was essentially a "waste of time" because he's done linkage mapping before, and when two genes don't complement, they are always the same gene. He wasn't even willing to consider the other option, even though the professor was unsure of the first possibility.

So seriously.. how does he know already? He hasn't even tried.
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Finally, I've noticed since grad school started I've became much more impatient about a lot of things outside of school. The program here (along with every other program I've interviewed at) expects grad school to be everyone's #1 priority. I think I'm a little scared of losing interest in what I'm doing if I lost focus, so I've channeled a lot of energy into learning what has been done in the past, what's going on now, and what new things might pop up in the future. Unfortunately that doesn't leave much room in my attention span, so everything else gets treated as a black and white issue.

I should probably take up yoga as a new pastime...